猪骨髓间充质干细胞体外诱导构建组织工程化软骨.pdfVIP

中国组织工程研究与临床康复 第12 .fi: 第2 柳 2008 - 0卜08 出版 Joumal ofClinical Rehabilirarive ηsSlIe Engineering R田earch January 8. 2008 均1. 12. NO .2 猪骨髓间充质干细胞体外诱导构建组织工程化软骨食 韩志军，刘晓邸，任华 Constructing tissue-engineered carti1age with porcine bone marrow mesenchymal stem cells in vitro Han Zhi才tJn， LitJ Xiao-zheng , Ren Hua Abstract 1M: Repair of trach ea is disturbing 由e surgeon . Tissue engi neering technology will probably resolve 由is problem. Seed cell is one Departmenl of ofthe key fàctors in engineered trachealωrtilage construction . This study investigated the feasi bility of constructing ti ssue-engineered Thoracic Surgery. cartilage 岳。m porcine bone maπow mesenchymal stem cell s (MSCs) cultured and induced ;11 vÎrro usiog ússue engineeriog technique . Pcking Union Medical Colle且e METHODS: The experiment was perform巳d in the Central Laboratory of Peking Un ion Mcdical College Hospi tal between OClobcr Hospilal. Chinese 2006 a nd May 2007 ( By d ensü y gradient cen甘i 仙gation， the MSCs w ere isolated and purified from porcine bone marrow . The Aca demy of Medi四l MSCs had been cultured and induced in the defined m edium mainly including transforming growth factor- 自 1, and then lhe typc 11 ScicncePcking Uni on Medical collagens were d etecred by immunohistochemical assay. The induced MSCs were seeded onto polyglycolic acids (PGA) scaffold as College. ßeij ing expe时mental group , aod PGA scaffold were implantωinto subcutaneous ti ssue as conlrol group. The cell-sca ffold con struct was 1