模拟急进高原环境下的小型猪创伤性脑出血模型的建立.docVIP

模拟急进高原环境下的小型猪创伤性脑出血模型的建立 朱海涛，张礼均，李飞，邹明明，袁轶恺，薛兴森，李 兰，冯 华，朱刚，林江凯 (400038重庆，第三军医大学西南医院神经外科，全军神经系统疾病微创诊治专科中心) 【摘要】 目的 建立模拟急进高原环境下的实验用小型猪创伤性脑出血的动物模型。 方法 用动物低压氧舱模拟高原环境，采用二次法缓慢注射3 ml自体动脉血于右侧基底节区建立脑出血模型，立即行头颅CT扫描，分别于注血后各时相点（2、24、72、168 h）连续监测动脉血血气、动物行为学，168 h后行脑组织大体切片并计算血肿体积、行HE、NisslA model of traumatic intracerebral hemorrhage in miniature pigs exposed to simulated high altitude Zhu Haitao， Zhang Lijun， Li Fei， Zou Mingming， Yuan Yikai， Xue Xingsen， Li Lan， Feng Hua， Zhu Gang， Lin Jiangkai (Department of Neurosurgery， Southwest Hospital， Third Military Medical UniversityObjective to establish a model of traumatic intracerebral hemorrhage (tICH) in miniature pigs exposed to simulated high altitude. Method 　Healthy miniature pigs were raised in hypobaric chamber simulating 4000 meter high altitude for 3days， then ICH was produced by a double-injection method in which 3ml of fresh autologous arterial blood was slowly injected into the right basal ganglia. CT was carried out immediately after tICH， then hematoma volume was calculated by stereology. Arterial blood gas and neurological behavior were monitered before tICH and after treatment (at 2h，， and 168h). 7 days after tICH， Brains were cut coronal sections for hematoma assessment， morphological brain examinationAt 24h， and 168h after tICH， there were signcant differences in blood gas analysis (p 0.01) and total neurological scores (p 0.01) between high altitude tICH group and plain tICH group. Injuries in caryon of neuron and tigroid bodies around the hematoma were more severe in high altitude tICH group than plain tICH group. Conclusion The model of tICH in miniature pigs exposed to simulated high altitude can produce stable hematoma volume and visible neurological deficits. [Keywords] high altitude；traumatic intracerebral hemorrhage；closed brain injury；miniature pig；animal model Supported by the Sci Tech Research Project of the“Eleventh Five-year Plan”for Medical Science Development of PLA（08G087）. Corresp