efficient transduction of feline neural progenitor cells for delivery of glial cell line-derived neurotrophic factor using a feline immunodeficiency virus-based lentiviral construct有效转导的猫科动物神经胶质细胞的神经祖细胞传递line-derived使用猫免疫缺陷virus-based慢病毒构建神经营养因子.pdfVIP

Hindawi Publishing Corporation Journal of Ophthalmology Volume 2011, Article ID 378965, 11 pages doi:10.1155/2011/378965 Research Article Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct X. Joann You,1 Ping Gu,1, 2 Jinmei Wang,1 Tianran Song,1 Jing Yang,1 Chee Gee Liew,3 and Henry Klassen1 1 Department of Ophthalmology, School of Medicine, Gavin Herbert Eye Institute, University of California, Irvine, 101 The City Drive, Bldg. 55, 2nd Fl, Orange, CA 92868-4380, USA 2 Department of Ophthalmology, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China 3 Department of Cell Biology and Neuroscience, Stem Cell Center, University of California, Riverside, Riverside, CA 92521, USA Correspondence should be addressed to Henry Klassen, hklassen@ Received 23 June 2010; Accepted 28 July 2010 Academic Editor: Muna Naash Copyright © 2011 X. Joann You et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Work has shown that stem cell transplantation can rescue or replace neurons in models of retinal degenerative disease. Neural progenitor cells (NPCs) modified to overexpress neurotrophic factors are one means of providing sustained delivery of therapeutic gene products in vivo. To develop a nonrodent animal model of this therapeutic strategy, we previously derived NPCs from the fetal cat brain (cNPCs). Here we use bicistronic feline lentiviral vectors to transduce cNPCs with glial cell-derived neurotrophic factor