cytotoxicity of silver nanoparticles in human embryonic stem cell-derived fibroblasts and an l-929 cell line细胞毒性的银纳米颗粒在人类胚胎干细胞的成纤维细胞和l - 929细胞系.pdfVIP

Hindawi Publishing Corporation Journal of Nanomaterials Volume 2012, Article ID 160145, 9 pages doi:10.1155/2012/160145 Research Article Cytotoxicity of Silver Nanoparticles in Human Embryonic Stem Cell-Derived Fibroblasts and an L-929 Cell Line Hui Peng,1 Xuehui Zhang,1 Yan Wei,1 Wentao Liu,1 Shenglin Li,2, 3 Guangyan Yu,4 Xin Fu,5 Tong Cao,5 and Xuliang Deng1 1 Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China 2 Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China 3 Laboratory of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China 4 Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China 5 Faculty of Dentistry Research Laboratories, National University of Singapore, Singapore 119083 Correspondence should be addressed to Xuliang Deng, kqdengxuliang@ Received 4 April 2012; Accepted 20 May 2012 Academic Editor: Xiaoming Li Copyright © 2012 Hui Peng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Consensus about the toxicity of silver nanoparticles (Ag-NPs) has not been reached, even though extensive attention has been paid to this issue. This confusion may be due to physicochemical factors of Ag-NPs and the cell model used for biological safety evaluation. In the present study, human embryonic stem cell-derived fibroblasts (EBFs), which have been considered a closer representative of the in vivo response, were used as a novel cell model to assess the cytotoxicity of Ag-NPs (∼20