close vicinity of prp expressing cells (fdc) with noradrenergic fibers in healthy sheep spleen关闭附近prp表达细胞(fdc)和去甲纤维在健康羊脾.pdfVIP

Developmental Immunology, 2001, Vol. 8(3-4), pp. 235-241 (C) 2001 OPA (OverseasPublishers Association) Reprints available directly from the publisher N.V. Published by license under Photocopying permitted by license only the Harwood Academic Publishers imprint, part of The Gordon and Breach Publishing Group, member of the Taylor and Francis Group Close Vicinity of PrP Expressing Cells (FDC)with Noradrenergic Fibers in Healthy Sheep Spleen A. BENCSIKa*, S. LEZMIa, G. HUNSMANNb and T. BARONa aAFSSA, Unit de Virologie-ATNC, 31 avenue Tony Garnier, BP 7033, 69342 Lyon cedex 07, France and bDeutsches Primatenzen- trum, Dept Virology and Immunology, D-37077 G6ttingen, Germany In naturally and experimentally occurring scrapie in sheep, prions invade the immune system and replicate in lymphoid organs. Here we analysed immunohistochemically, in seven spleens of 6-month-old healthy sheep, the nature of the cells expressing prion protein (PrP) poten- tially supporting prion replication, as well as their relationship with autonomic innervation. PrP was identified using either RB rabbit antiserum or 4F2 monoclonal antibody directed against AA 108-123 portion of the bovine and AA 79-92 of human prion protein respec- tively. Using double labelling analysis, we demonstrated that PrPc is expressed by follicular dendritic cells using a s