expression of tlr4-myd88 and nf- b in the iris during endotoxin-induced uveitistlr4-myd88表达式和nf - b在虹膜endotoxin-induced葡萄膜炎.pdfVIP

Hindawi Publishing Corporation Mediators of Inflammation Volume 2010, Article ID 748218, 7 pages doi:10.1155/2010/748218 Research Article Expression of TLR4-MyD88 and NF-κB in the Iris during Endotoxin-Induced Uveitis Shang Li,1 Hong Lu,1 Xiaofeng Hu,1 Wei Chen,2 Yingzhi Xu,1 and Jing Wang1 1 Departmnet of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Baijiazhuang Road, Chaoyang District, Beijing 100020, China 2 Department of Ophthalmology, Haidian Maternal and Child Health Hospital, Tonji Medical University, Beijing 100080, China Correspondence should be addressed to Hong Lu, honglu111@ Received 25 November 2009; Accepted 21 June 2010 Academic Editor: Philipp M. Lepper Copyright © 2010 Shang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose. To observe the expression of Toll-like receptor-4 (TLR4), myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B p65 (NF-κB p65) in iris tissue during endotoxin-induced uveitis (EIU) and evaluate the significance of these factors in uveitis. Methods.Wistar rats were randomly divided into 5 groups (0 h, 12 h, 24 h, 48 h, and 72 h, n = 10/group). Animal model of acute anterior uveitis was established by a hind footpad injection of 200 μg Cholera vibrio LPS. Expression of TLR4, MyD88, and NF-κB p65 in iris ciliary body tissue was detected through immunohistochemical staining. Results. Expression of TLR4 was not detected in normal iris-ciliary body complex, TLR4 positive cells with round morphology appeared in the iris stroma 12 hours after injection, significantly increased (P .001) 48 hours aft