binding of hoechst with nucleic acids using fluorescence spectroscopy使用荧光光谱结合核酸的赫斯特.pdfVIP

Vol.2, No.4, 443-447 (2011) Journal of Biophysical Chemistry doi:10.4236/jbpc.2011.24052 Binding of Hoechst with nucleic acids using fluorescence spectroscopy Nikolai Vekshin Institute of Cell Biophysics of Russian Academy of Sciences, Pushchino, Moscow Region, Russia. nvekshin@rambler.ru Received 2 August 2011; revised 14 September 2011; accepted 27 September 2011. ABSTRACT Full understanding of the molecular mechanisms of action of heterocyclic antibiotics at low concentrations It has been shown that polarity of environment on DNA and RNA can optimize the use of these com- around Hoechst 33342 is almost unchanged pounds and their analogues as medical drugs. while sorption of this fluorescent dye on a sur- Hoechst dyes 33342 and 33258 are closely related bis- face of the hairpin oligonucleotide HP1, t-RNA benzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl)-5- and DNA. At small concentrations, this dye, ad- sorbed on the surface of DNA, RNA or HP1, does [5-(4-methylpiperazine-1-yl) benzimidazo-2-yl]-benzimi- not show any specificity to certain nucleotides. dazole. Hoechst 33342 contains additional ethyl group. In the case of unwound sites of DNA or HP1, it This group renders Hoechst 33342 a bit more hydropho- can bind inside, but without the intercalation bic than Hoechst 33258. Both