biosynthesis of ethanol and hydrogen by glycerol fermentation using escherichia coli生物合成甘油发酵乙醇和氢气的使用大肠杆菌.pdfVIP

Advances in Chemical Engineering and Science, 2011, 1, 83-89 doi:10.4236/aces.2011.13014 Published Online July 2011 (http://www.SciRP.org/journal/aces) Biosynthesis of Ethanol and Hydrogen by Glycerol Fermentation Using Escherichia coli 1 1 2 3 Nida Chaudhary , Michael O. Ngadi , Benjamin K. Simpson , Lamin S. Kassama 1 Department of Bioresource Engineering, McGill University, Montreal, Canada 2Department of Food Science, McGill University, Montreal, Canada 3Department of Food and Animal Sciences, Alabama AM University, Huntsville, USA E-mail : michael.ngadi@mcgill.ca Received May 13, 2011; revised June 20, 2011; accepted June 28, 2011 Abstract Production of high value products from glycerol via anaerobic fermentation is of utmost importance for the biodiesel industry. The microorganism Escherichia coli (E. coli) K12 was used for fermentation of glycerol. The effects of glycerol concentration and headspace conditions on the cell growth, ethanol and hydrogen production were investigated. A full factorial experimental design with 3 replicates was conducted in order to test these factors. Under the three headspace conditions tested, the increase of glycerol concentration ac- celerated glycerol fermentation. The yields of hydrogen and ethanol were the lowest when glycerol concen- tration of 10 g/L was used. The maximum production of hydrogen was observed with an initial glycerol concentration of 25 g/L at a final concentration of hydrogen was 32.15 mmol/L. This study demonstrated that hydrogen production negatively affects cell growth. Maximum et