原创力文档characterization of a collection of brassica carinata genotypes for in vitro culture response and mode of shoot regeneration芸苔属植物的特征集合的carinata基因型体外文化反应和拍摄方式再生.pdfVIP
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characterization of a collection of brassica carinata genotypes for in vitro culture response and mode of shoot regeneration芸苔属植物的特征集合的carinata基因型体外文化反应和拍摄方式再生
American Journal of Plant Sciences , 2011, 2, 27-34 27
doi:10.4236/ajps.2011.21003 Published Online March 2011 (http://www.SciRP.org/journal/ajps)
Characterization of a Collection of Brassica
carinata Genotypes for in vitro Culture Response
and Mode of Shoot Regeneration
Javier Gil-Humanes, Antonio Martín, Francisco Barro
Institute for Sustainable Agriculture, CSIC. E-14080, Córdoba, Spain.
Email: javigil@ias.csic.es
Received August 5th st th
, 2010; revised September 21 , 2010; accepted September 28 , 2010.
ABSTRACT
Brassica carinata, a natural alloploid formed between B. oleracea and B. nigra, is a potential oil crop for the Mediter-
ranean area in which genetic transformation could help to breed. In vitro culture and shoot regeneration are key fac-
tors in developing an efficient transformation method in the genus Brassica. However, the studies for in vitro culture
and shoot regeneration in B. carinata are limited to only a few genotypes. The aim of this study was to evaluate the in
vitro culture response and shoot regeneration in a collection of B. carinata accessions to identify promising genotypes
with high shoot regeneration for genetic transformation experiments. Cotyledonary explants from 51 genotypes were
cultured in vitro and callus formation and swelling as well as the mode of shoot regeneration evaluated. A highly posi-
tive response to in vitro culture, i.e. callus formation or swelling, was observed in all the genotypes tested. Tissue
blackening occurred only in eleven genotypes. Parameters like callus formation and swelling and number of shoots per
explant were highly variable among genotypes. Fourteen genotypes regenerated only via callus formation, whereas
only one regenerated only via swelling. Mo
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