characterization of newly developed wheatbarley introgression lines in respect of aluminium tolerance描述新开发wheatbarley基因渗入线的铝宽容.pdfVIP

American Journal of Plant Sciences, 2012, 3, 1462-1469 /10.4236/ajps.2012.310176 Published Online October 2012 (http://www.SciRP.org/journal/ajps) Characterization of Newly Developed Wheat/Barley Introgression Lines in Respect of Aluminium Tolerance 1* 1 2 Eva Darko , Beáta Barnabás , Márta Molnár-Láng 1Cell Biology Department of Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2 Hungary; Department of Plant Genetic Resources and Organic Breeding of Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary. * Email: edarko@mail.mgki.hu Received July 25th, 2012; revised August 22nd, 2012; accepted September 2nd, 2012 ABSTRACT The Al tolerance of newly developed wheat/barley disomic addition, substitution and translocation lines carry chromo- somes of three different barley cultivars was evaluated by comparing the root growth in solution containing 75 μM AlCl3 at pH 4.0 to that of known Al-tolerant and sensitive wheat genotypes. The wheat Asakaze komugi, barley Manas cultivars and their hybrid derivatives were found to have high levels of Al tolerance. The wheat line Mv9kr1, barley cultivar Igri and progenies of the hybrids were sensitive to Al. In most cases, the Al tolerance of the wheat/barley intro- gression lines derived from Al-sensitive wheat Mv9kr1 and barley Betzes with moderate Al tolerance was similar to that of the wheat parents, but the 2DS.2DL-1HS translocation line of Mv9kr1/Betzes exhibited more intensive root growth, while accumulating less Al than the parental lines. This indicates that either the lack of the distal part of chro- mosome 2DL or the presence of the distal part of 1HS improved the Al tolerance level. Keywords: Aluminium Tolerance; Barley; Introgressi