原创力文档constrain-based analysis of gene deletion on the metabolic flux redistribution of saccharomyces cerevisiaeconstrain-based分析基因缺失在酿酒酵母的代谢通量再分配.pdfVIP
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constrain-based analysis of gene deletion on the metabolic flux redistribution of saccharomyces cerevisiaeconstrain-based分析基因缺失在酿酒酵母的代谢通量再分配
J. Biomedical Science and Engineering, 2008, 1, 121-126
Constrain-based analysis of gene deletion on the
metabolic flux redistribution of Saccharomyces
Cerevisiae
Zi-Xiang Xu Xiao Sun
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China. Correspondence should be addressed to Z. X. Xu.( xzx21c@163.com,
xzxnpu@).
ABSTRACT stochastic and cybernetic approaches, are currently being
used to model cellular processes. Owing to the computa-
Based on the gene-protein-reaction (GPR) tional complexity and the large number of parameters
model of S. cerevisiae_iND750 and the method needed, it is currently difficult to use these methods to
of constraint-based analysis, we first calculated model genome-scale networks. To date, genome-scale
the metabolic flux distribution of S. cere- models of metabolism have only been analyzed with the
visiae_iND750. Then we calculated the deletion constraint-based modeling philosophy [2, 3]. Ge-
impact of 438 calculable genes, one by one, on nome-scale network models of diverse cellular processes
the metabolic flux redistribution of S. cere- such as signal transduction, transcriptional regulation and
8 visiae_iND750. Next we analyzed the correlation metabolism have been generated. Gene-protein-reaction
0 between v (describing deletion impact of one (GPR) associated models can keep track of associations
0
2
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