cytotoxicity of pristine c60 fullerene on baby hamster kidney cells in solution细胞毒性的原始c60富勒烯在婴儿仓鼠肾细胞的解决方案.pdfVIP

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cytotoxicity of pristine c60 fullerene on baby hamster kidney cells in solution细胞毒性的原始c60富勒烯在婴儿仓鼠肾细胞的解决方案.pdf

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cytotoxicity of pristine c60 fullerene on baby hamster kidney cells in solution细胞毒性的原始c60富勒烯在婴儿仓鼠肾细胞的解决方案

Journal of Biomaterials and Nanobiotechnology, 2012, 3, 385-390 385 /10.4236/jbnb.2012.33037 Published Online July 2012 (http://www.SciRP.org/journal/jbnb) Cytotoxicity of Pristine C60 Fullerene on Baby Hamster Kidney Cells in Solution * Shufang Liu, Haijie Liu, Zhijuan Yin, Kai Guo, Xibao Gao School of Public Health, Shandong University, Jinan, China. * Email: chem@ Received May 23rd, 2012; revised June 25th, 2012; accepted July 2nd, 2012 ABSTRACT Research on biological effects of C60 and its derivatives is one of the hotspots in the area of biological effects of nano- materials. Compared with surface-modified C60 derivatives, reports on the biological effects of unmodified pristine C60 are relatively less. This work aimed to investigate the interaction between baby hamster kidney cells and pristine C60 in solution. The C60 suspension was prepared using solvent exchange method and characterized by UV spectro-photometry, electronic transmission microscopy and dynamic light scattering techniques. The baby hamster kidney cells were incu- bated with different concentrations of C60 suspensions, and light microscopy, cell counting kit 8 assay, and acridine orange staining were used to observe the cell growth and morphology. The results showed that C60 could inhibit the cell growth and induce cell apoptosis with a dose-effect relationship. C60 might enter cells and the possible way it enters cells were also proposed. Keywords: C60; Cell; Cytotoxicity; Fullerene; Toxicity 1. Introduction tosis [15]. They mainly deposited in the cytoplasm, nu- cleus, lysosome [14,16], cell membrane [17] and