dietary karaya saponin and rhodobacter capsulatus exert hypocholesterolemic effects by suppression of hepatic cholesterol synthesis and promotion of bile acid synthesis in laying hens英文论文.pdfVIP

Hindawi Publishing Corporation Cholesterol Volume 2010, Article ID 272731, 7 pages doi:10.1155/2010/272731 Research Article Dietary Karaya Saponin and Rhodobacter capsulatus Exert Hypocholesterolemic Effects by Suppression of Hepatic Cholesterol Synthesis and Promotion of Bile Acid Synthesis in Laying Hens Sadia Afrose, Md. Sharoare Hossain, Ummay Salma, Abdul Gaffar Miah, and Hirotada Tsujii Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shinshu University, Minamiminowa-mura, Nagano 399-4598, Japan Correspondence should be addressed to Hirotada Tsujii, htsujii@shinshu-u.ac.jp Received 27 October 2009; Revised 18 February 2010; Accepted 17 May 2010 Academic Editor: Philippe G. Frank Copyright © 2010 Sadia Afrose et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This study was conducted to elucidate the mechanism underlying the hypolipidemic action of karaya saponin or Rhodobacter (R.) capsulatus. A total of 40 laying hens (20-week-old) were assigned into four dietary treatment groups and fed a basal diet (as a control) or basal diets supplemented with either karaya saponin, R. capsulatus, or both for 60 days. The level of serum low-density-lipoprotein cholesterol and the levels of cholesterol and triglycerides in the serum, liver, and egg yolk were reduced by all the supplementations (P .05). Liver bile acid concentration and fecal concentrations of cholesterol, triacylglycerol, and bile acid were simultaneously increased by the supplementation of karaya saponin, R. capsulatus, and the combination of karaya saponin and R. capsulatus (P .05).