聚羟基丁酸酯可吸收缝线生物相容性实验室探究.docVIP

聚羟基丁酸酯可吸收缝线生物相容性实验室探究[摘要]目的:研究聚羟基丁酸酯(Polyhydroxybutyrate,PHB)可吸收缝线的生物相容性。方法:①将成纤维细胞进行体外培养,传代后与PHB可吸收缝线复合培养,设与经聚氰基丙烯酸酯处理过的PHB可吸收缝线复合培养为对照组,观察细胞生长情况;②行扫描电镜检查观察细胞在丝线上的生长情况;③以MTT法检测复合培养后的细胞活性。结果:①实验组细胞生长良好,对照组细胞漂浮,收缩成团;②扫描电镜下见细胞在丝线上贴附生长良好;③MTT法检测实验组对细胞增殖无明显影响。结论:PHB可吸收缝线具有良好的生物相容性。 [关键词]可吸收缝线; 聚羟基丁酸酯;成纤维细胞 [中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2010)04-0531-03 Investigation of the biocompatibility of polyhydroxybutyrate absorbable suturein laboratory WU Jin-hua1,LIU Bin1,AI Yu-feng2,YANG Qing-fang3,DONG Zhao-lin4 (1.Department of Burn and Plastic, Xi’an Central Hospital,Xi’an 710003,Shaanxi,China; 2.Sichuan Huamei Zixin Medical Cosmetology Hospital; 3.Polymer materials,Northwestern Polytechnical University University; 4.College of Life Science, Northwest University) Abstract:ObjectiveTo investigate the biocompatibility of absorbable suture made of Polyhydroxybutyrate (PHB).MethodsThe fibroblast were co-cultured with PHB suture verse fibroblast co-cultured with PHB suture which had been dipped in the cyanoacrylate sdhesive(for cytotoxicity),the proliferation and the adhering conditionare observed by inverted microscope and scanning electronic microscope,Culture fibroblast inculture plates containing PHB suture,investigate the attachment rate with MTT assay.ResultsThe number of fibroblastincreased co-cultured with PHB,and the comparison cellswere floated and shrinked,by scanning electronic microscope,there were cellsconnect each other adhering on the surface ofthe suture.ConclusionPHB have fine biocompatibility with fibroblast. Key words: absorb suture;polyhydroxybutyrate;fibroblast 1 材料和方法 1.1 试件:实验所用PHB可吸收缝线为西北大学生命科学院和西北工业大学理学院应用化学系联合研制,分子量为1.5×105～2.0×105,纯度99%,菌种为园褐固氮菌G-3,纤维纤度0.04～0.18dtex。 1.2 仪器:BB16型CO2培养箱 (Heraeus,上海),SW-CJ-1B型超净工作台( 苏净集团),倒置显微镜(Olympus,Japan),低温冰箱( SANYO),0.22μm、0.45μm微孔滤膜(Sigma),S-520扫描电镜(HITACHI,Japan), DG5031型酶联免疫检测仪,LG10-3A低温高速离心机(北京医用离心机厂)。 1.3 试剂:小牛血清(FBS杭州四季青生物有限公司),DMEM培养液 (GIBCO公司),胰蛋白酶 (Sigma),MTT(Sigma),b