disease development caused by ascochyta rabiei on chickpea detached-leaves in petri dishes疾病发展引起的壳二孢rabiei鹰嘴豆detached-leaves培养皿.pdfVIP

American Journal of Plant Sciences, 2012, 3, 1369-1375 1369 /10.4236/ajps.2012.310165 Published Online October 2012 (http://www.SciRP.org/journal/ajps) Disease Development Caused by Ascochyta rabiei on Chickpea Detached-Leaves in Petri Dishes Nunung Harijati1*, Philip J. Keane2 1Department of Biology, Brawijaya University, Malang, Indonesia; 2Department of Botany, LaTrobe University, Melbourne, Austra- lia. * Email: harijati@ub.ac.id th rd st Received August 26 , 2012; revised September 23 , 2012; accepted October 1 , 2012 ABSTRACT A study using detached-leaves aimed to improve selection method. The improving method was done by scoring both disease symptom and lesion size. The research was begun by selecting agar concentration and dose of conidia that could distinguish response of very susceptible or resistant chickpea genotype. The result was used to determine disease sever- ity (DS) and disease incident (DI) of eight genotypes that were previously tested in the field. Results of the tested agar concentration and dose of conidia showed that 1.5% and 2% agar were good to determine susceptible or resistant geno- 5 4 type; while 1 × 10 or 5 × 10 conidia dose was suitable for inoculation. The formula of DS (no. of leaflets in category × category value/Total no. of leaflets × 10) × 100, and DI (no. leaflets with pycnidial lesions + no. leaflets with necrotic lesions)/Total no. of leaflets × 100 successfully measured genotype response. The lesions development on detached leaves of the susceptible cultivar (Lasseter) began as circular, pale-colored areas, extending to the area covered by the drop of inoculum, then became light brown and finally dark brown. However, the response of resistant line