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effect of growth medium ph of aeropyrum pernix on structural properties and fluidity of archaeosomes影响生长介质的ph值的aeropyrum pernix archaeosomes的结构性能和流动性.pdf

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effect of growth medium ph of aeropyrum pernix on structural properties and fluidity of archaeosomes影响生长介质的ph值的aeropyrum pernix archaeosomes的结构性能和流动性

Hindawi Publishing Corporation Archaea Volume 2012, Article ID 285152, 9 pages doi:10.1155/2012/285152 Research Article Effect of Growth Medium pH of Aeropyrum pernix on Structural Properties and Fluidity of Archaeosomes 1 1 2 1, 3 ˇ ˇ Ajda Ota, Dejan Gmajner, Marjeta Sentjurc, and Natasa Poklar Ulrih 1 Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia 2 EPR Center, Institute Jozef Stefan, Jamova 39, 1000 Ljubljana, Slovenia ˇ 3 Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CipKeBiP), Jamova 39, 1000 Ljubljana, Slovenia ˇ Correspondence should be addressed to Natasa Poklar Ulrih, natasa.poklar@bf.uni-lj.si Received 11 January 2012; Revised 30 March 2012; Accepted 13 April 2012 Academic Editor: Parkson Chong Copyright © 2012 Ajda Ota et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The inﬂuence of pH (6.0; 7.0; 8.0) of the growth medium of Aeropyrum pernix K1 on the structural organization and ﬂuidity of archaeosomes prepared from a polar-lipid methanol fraction (PLMF) was investigated using ﬂuorescence anisotropy and electron paramagnetic resonance (EPR) spectroscopy. Fluorescence anisotropy of the