芹菜素对大鼠脑缺血再灌注NF-κBiNOS动态表达影响.docVIP

芹菜素对大鼠脑缺血再灌注NF-κBiNOS动态表达影响摘 要：目的:探讨芹菜素对局灶性脑缺血再灌注损伤后核转录因子-κB（NF-κB）、诱导型一氧化氮合酶（iNOS）动态表达的影响。方法:将大鼠135只随机分为假手术组（S组）、模型组（M组）及芹菜素组（A组），后2组按再灌注时间不同又分为再灌注24h、48h、72h和7天各4小组，总共9小组，每组15只大鼠。采用线栓法制备大鼠左侧大脑中动脉缺血（1.5h）再灌注模型。光镜电镜观察脑组织病理形态改变，免疫组织化学法检测NF-κBp65蛋白表达，比色法检测iNOS活性。结果:大鼠脑缺血再灌注后M组各时间点NF-κBp65表达增多（P0.01）、iNOS活性增高（P0.01）。芹菜素在脑缺血再灌注后72h和7天有下调NF-κBp65的表达（P0.05），降低iNOS活性的作用（P0.05）。M组脑缺血再灌注后各个时间点皮层和海马细胞肿胀、细胞间质水肿，有空泡形成，尤以缺血再灌注后48h最为明显，而神经细胞均有核固缩、碎裂、溶解，以缺血再灌注后24h为著。芹菜素有减轻脑缺血再灌注后组织细胞水肿的作用，对神经细胞超微结构改变不明显。结论:芹菜素的神经保护作用可能与抑制大鼠缺血再灌注后脑组织中NF-κB的表达及iNOS活性有关。? 关键词：缺血再灌注;核转录因子-κB;诱导型一氧化氮合酶;芹菜素? 中图分类号：R285.5文献标识码：A 文章编号：1673-7717(2008)11-2438-04? Effect of Apigenin on the Expression of NF-κB and iNOS in Transient? Focal Cerebral Ischemia and Reperfusion Model of Rats ? TU Feng?xia,LIU Chan，CHEN Xiang，LIN Xiao?yan,ZHANG Shuang,LIANG Yan?ling? (The Second Affiliated Hospital of Wenzhou Medical College，Wenzhou 325027，Zhejiang,China)? Abstract:Objective：To study the neuroprotective effect of apigenin on transient focal cerebral ischemia and reperfusion injury in rats,which was characterized by its influence on nuclear factor-κappaB (NF-κB) and inducible nitric oxide synthase (iNOS) expression. Methods：One hundred and thirty five male Spragur-Dawley rats were randomized into 9 groups :sham-operated group(S,n=15); model groups(group M24h,group M48h,group M72h,group M7d,n=15)and apigenin-treated groups(group A?24h,group A?48h,group A72h,grouop A7d,n=15).The transient focal cerebral ischemia and reperfusion(1.5hours) model in rat was established with insertion of thread embolish into middle cerebral artery. The cellular and subcellular morphology under optic microscope and electromicroscope, the expression of NF-κB p65 by immuno-histochemistry (ICH ) and the activity of inducible nitric oxide synthase (iNOS) by colorimetric method were measured.Results：The expression of NF-κB p65 were significantly higher in group M (P0.01) , the positive expressions of NF-κB p65 in