effect of the monocyte locomotion inhibitory factor (mlif) a natural anti-inflammatory produced by e. histolytica on apoptosis in human cd4 + t lymphocytes单核细胞运动的影响抑制因子(mlif)产生的一种天然的抗炎大肠阿米巴在人类cd4 + t淋巴细胞凋亡.pdfVIP

下载本文档

9
0
约4.55万字
约 8页
2017-08-28 发布于上海
举报
版权申诉

effect of the monocyte locomotion inhibitory factor (mlif) a natural anti-inflammatory produced by e. histolytica on apoptosis in human cd4 + t lymphocytes单核细胞运动的影响抑制因子(mlif)产生的一种天然的抗炎大肠阿米巴在人类cd4 + t淋巴细胞凋亡.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

effectofthemonocytelocomotioninhibitoryfactor(mlif)anaturalanti-inflammatoryproducedbye.histolyticaonapoptosisinhumancd4tlymphocytes单核细胞运动的影响抑制因子(mlif)产生的一种天然的抗炎大肠阿米巴在人类cd4t淋巴细胞凋亡

Pharmacology Pharmacy, 2011, 2, 248-255 doi:10.4236/pp.2011.24032 Published Online October 2011 (http://www.SciRP.org/journal/pp) Effect of the Monocyte Locomotion Inhibitory Factor (MLIF) a Natural Anti-Inflammatory Produced by E. histolytica on Apoptosis in Human CD4+ T Lymphocytes 1 2 1 Sara Rojas-Dotor , Liliana Vargas-Neri , Francisco Blanco-Favela 1Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI Instituto Mexicano del Seguro Social, México City, México; 2Facultad de Química, Universidad Nacional Autónoma de México, México City, México. Email: srdotor@.mx Received June 4th, 2011; revised July 28th, 2011; accepted August 16th, 2011. ABSTRACT Apoptosis prevents the extravasation of intracellular material and the subsequent inflammatory response. Currently , it is not known whether Monocyte Locomotion Inhibitor Factor (MLIF), an anti-inflammatory pentapeptide, induces pro- grammed cell death. We evaluated the effect of MLIF on extrinsic and intrinsic apoptosis pathways human CD4+ T lymphocytes. Cells were cultured for 24 h in RPMI-1640 medium alone (control) or in RPMI medium containing MLIF alone, PMA alone, PMA + MLIF or actinomycin D. Annexin V/propidium iodide-stained cells in early apoptosis showed that cells treated with MLIF or PMA + MLIF were not significantly different from control cells in medium; in contrast, cells treated with PMA or PMA + MLIF demonstrated significant differences from the control in delayed apoptosis. Cytochrome c and caspase 3 levels in cells treated with MLIF showed no significant differences from control cells, however, compared to the control, cells treated with PMA and PMA + MLIF exhibited a significant increase in cytochrome c and caspase 3 levels, which demonstrates that this weak induction of cell death is re