软坚消瘿汤治疗实验性自身免疫性甲状腺炎疗效机制探究.doc

软坚消瘿汤治疗实验性自身免疫性甲状腺炎疗效机制探究收稿日期:2009-11-21 基金项目:辽宁省教育厅资助项目 作者简介:张兰(1962-),女,教授、主任医师,研究方向:中医药防治甲状腺疾病疗效及机理研究。 摘 要:目的:观察软坚消瘿汤对实验性自身免疫性甲状腺炎(EAT)大鼠的自身抗体、白介素-1、4、6及肿瘤坏死因子-α和甲状腺形态学的影响,从多靶点探讨其作用机制。方法:运用甲状腺球蛋白与弗氏佐剂混合免疫注射法,联合饮用高碘水模拟大鼠实验性自身免疫性甲状腺炎。酶联免疫法检测甲状腺抗体、白介素-1、4、6及肿瘤坏死因子-α水平,HE染色法观察大鼠甲状腺病理改变。结果:软坚消瘿汤能显著降低EAT大鼠自身抗体及相关细胞因子水平,减轻甲状腺滤泡损伤。结论:软坚消瘿汤通过降低EAT血清中自身抗体及细胞因子水平起到减轻甲状腺病理损伤的作用。 关键词:软坚消瘿汤;实验性自身免疫性甲状腺炎;血清抗体;细胞因子;中医实验医学? 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0843-03 Effect Mechanism of Ruanjian Xiaoying Decoction on Experimental Autoimmune Thyroiditis ZHANG Lan,FANG Zhen?wei ( The First Affiliated Hospital of Liaoning University of Chinese Medicine,Shenyang 110032,Liaoning,China)? Abstract:?Objective?:To evaluate the therapeutic effect of Xiaoying Ruanjian Decoction on experimental autoimmune thyroiditis (EAT) from the levels of autoantibodies, interleukin-1,4,6 and tumor necrosis factor-α and the thyroid morphology of EAT rats.To Investigate the effect Mechanism from multiple target point.?Methods?:An animal model of experimental autoimmune thyroiditis ( EAT) in rats was developed by injecting thyroglobulin mixed with Freund’s adjuvant immunization and drinking Periodate water. To detect the levels of autoantibodies, interleukin-1,4,6 and tumor necrosis factor-α with Enzyme-linked immunosorbent assay.To observe the pathological change in the thyroid of rat with HE staining.?Results?:Ruanjian Xiaoying Decoction significantly reduces the levels of autoantibody, cytokines and the damage of thyroid follicular.?Conclusion?:Ruanjian Xiaoying Decoction alleviates the pathological injury of thyroid by the suppression of relative expressions of antibodies and cytokines in serum levels.? Key words:Ruanjian Xiaoying Decoction; experimental autoimmune thyroiditis; serum antibody; cytokine; TCM Experimental Medicine 自身免疫性甲状腺炎是常见甲状腺病之一,目前尚缺乏有效治疗措施。软坚消瘿汤是笔者运用中医药理论,总结前辈及自己多年治疗经验创立的治疗本病的中药复方。此方具疏肝行气解郁、活血软坚散结之功,前期临床疗效甚好。早期动物实验研究揭示了其治疗EAT的部分机理,本实