黄芪延缓小鼠皮肤衰老实验探究.docVIP

黄芪延缓小鼠皮肤衰老实验探究[摘要]目的:从形态学角度研究黄芪延缓小鼠皮肤衰老的作用。方法:小鼠随机分成3组,空白组(C)灌胃生理盐水20ml/kg,黄芪低剂量组(L)和黄芪高剂量组(H)分别灌胃黄芪提取液4g/kg、16g/kg,每次20ml/kg。30天后做组织学切片检测。结果:与C组比较,L组和H组皮肤组织中真皮厚度和胶原纤维面积增加(P0.01)。H组与L组比较,真皮厚度和胶原纤维面积显著增加(P0.05)。结论:高剂量黄芪可使真皮厚度和胶原纤维面积明显增加,具有延缓小鼠皮肤衰老的作用。 [关键词]皮肤;衰老;黄芪 [中图分类号]R322.99[文献标识码]A[文章编号]1008-6455(2010)02-0209-03 Experimental study of the effects of Astragali on delaying skin aging in mices CONG Jing1,WU Jing-dong2,SU Qiu-xiang3 (1.Department of Histology-Embryology, Shenyang Medical College,Shenyang 110034, Liaoning,China;2.Liaoning University of Traditional Chinese Medicine Shenyang 110031, Liaoning,China;3.Morphology Central Lab, Shenyang Medical College) Abstract:ObjectiveTo investigate the effects of Astragali on delaying skin aging in mices from morphology point of view. Methods 60 mices were selected and randomly divided into 3 groups: normal control group (group C), low dose group (group L) and high dose group (group H). In group C, normal saline (20ml/kg) was given intragastricly. Astragali extracts at doses of 4g/kg and 16g/kg were administered intragastricly in group L and group H respectively. Histological sections of skin from treated mices were prepared for analysis 30 days after treatments. Results Increases in dermis thickness and collageous fibers area were observed in both group L and group H as compared with group C (P0.01). Compared with mice in group L, mices in group H showed significant increases in dermis thickness and collageous fibers area (P0.05). Conclusions Intragastric administration of Astragali extracts to mice could lead to increases in dermis thickness and collageous fibers area in mice skin, and therefore could delay the skin aging in mices. Key words: skin; aging; Astragali 衰老是一个伴随着时间的消逝构成有机体的所有组织逐渐发生结构和功能改变的生物过程[1]。皮肤松弛、皱纹和老年斑等临床症状的出现,使人们开始意识到衰老,从而引发一系列的心理问题和社会问题。因此,预防和延缓皮肤衰老一直是医学美容研究的热点和焦点。黄芪是中医传统的益气药,具有补气升阳、益卫固表、利水消肿、托疮生肌等作用。现代药理学研究表明,黄芪含有多种有效成分,具有增强机体免疫机能、调节血糖、降压利尿、保肝保肾、抗炎、抗菌、抗突变等作用[2]。本研究探讨了内服黄芪是否能