bone marrow derivation of interstitial cells of cajal in small intestine following intestinal injury骨髓来源的间质细胞,卡哈尔在小肠肠道损伤.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2010, Article ID 164986, 9 pages doi:10.1155/2010/164986 Research Article Bone Marrow Derivation of Interstitial Cells of Cajal in Small Intestine Following Intestinal Injury Dengqun Liu, Fengchao Wang, Zhongmin Zou, Shiwu Dong, Junping Wang, Xinze Ran, Chunxue Li, Chunmeng Shi, and Yongping Su State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China Correspondence should be addressed to Yongping Su, suyp2003@ Received 1 September 2009; Revised 24 December 2009; Accepted 27 January 2010 Academic Editor: Ronnie Gallagher Copyright © 2010 Dengqun Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Interstitial cells of Cajal (ICCs) in gastrointestinal tract are specialized cells serving as pacemaker cells. The origin of ICCs is currently not fully characterized. In this work, we aimed to study whether bone marrow-derived cells (BMDCs) could contribute to the origin of ICCs in the muscular plexus of small intestine using GFP-C57BL/6 chimeric mice.Engraftment of BMDCs in the intestine was investigated for GFP expression. GFP positive bone marrow mononuclear cells reached a proportion of 95.65% ±3.72% at different times in chimerism. Donor-derived cells distributed widely in all the layers of the gastrointestinal tract. There were GFP positive BMDCs in the myenteric plexus, which resembled characteristics of ICCs, including myenteric location, c-Kit positiv