repertoire of ssrs in the castor bean genome and their utilization in genetic diversity analysis in jatropha curcas曲目蓖麻子ssrs的基因组及其利用率在麻风树的遗传多样性分析.pdf

Hindawi Publishing Corporation Comparative and Functional Genomics Volume 2011, Article ID 286089, 9 pages doi:10.1155/2011/286089 Research Article Repertoire of SSRs in the Castor Bean Genome and Their Utilization in Genetic Diversity Analysis in Jatropha curcas Arti Sharma and Rajinder Singh Chauhan Department of Biotechnology Bioinformatics, Jaypee University of Information Technology, Waknaghat, P.O. Dumehar Bani, Kandaghat, Solan 173 215, India Correspondence should be addressed to Rajinder Singh Chauhan, rajinder.chauhan@rediff Received 9 August 2010; Revised 16 February 2011; Accepted 9 March 2011 Academic Editor: Neil Hall Copyright © 2011 A. Sharma and R. S. Chauhan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Castor bean and Jatropha contain seed oil of industrial importance, share taxonomical and biochemical similarities, which can be explored for identifying SSRs in the whole genome sequence of castor bean and utilized in Jatropha curcas . Whole genome analysis of castor bean identified 5,80,986 SSRs with a frequency of 1 per 680 bp. Genomic distribution of SSRs revealed that 27% were present in the non-genic region whereas 73% were also present in the putative genic regions with 26% in 5UTRs, 25% in introns, 16% in 3 UTRs and 6% in the exons. Dinucleotide repeats were more frequent in introns, 5 UTRs and 3 UTRs whereas trinucleotide repeats were predominant in the exons. The transferability of randomly selected 302 SSRs, from castor bean to 49 J.