secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa分泌白细胞蛋白酶抑制剂在冲头从人类结肠粘膜活检.pdf

Short Communication Mediators of Inflammation, 10, 269 –272 (2001) SE C RE T O RY leukocyte protease inhibitor (SLPI) is a well- Secretory leukocyte protease known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteo- inhibitor in punch biopsies from lytic activity, mainly pancreatic elastase, anionic human colonic mucosa trypsin and granulocytic elastase, has been demon- strated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, 1,2,CA 2 Max Nystrom¨ , Ulla Peterson Westin , we wanted to ascertain whether studies of SLPI 2 2 Carina Linder and Kjell Ohlsson secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four 1Department of Surgery and 2 Department of patients were used. Mucosa preparation was carried Surgical Pathophysiology, Ing 37, Lund University, out using a punch biopsy technique, and samples of 3, University Hospital Malmo,¨ SE 20502 Malmo,¨ 4 and 6 mm diameter were used. All media contained Sweden SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per milli- metre-squared seemed to decrease with increasing area. When calculated as secretion per circumfer- CA Corresponding Author ence, secretion seemed to be constant. In conclusion, Tel: +46 40 33 31 50 SLPI was secreted fro