site-specific insertion polymorphism of the mite alex-1 in the genus coffea suggests interspecific gene flow特定站点插入多态性的螨虫alex-1属coffea表明种间基因流动.pdf

SAGE-Hindawi Access to Research International Journal of Evolutionary Biology Volume 2011, Article ID 358412, 9 pages doi:10.4061/2011/358412 Research Article Site-Specific Insertion Polymorphism of the MITE Alex-1 in the Genus Coffea Suggests Interspecific Gene Flow Christine Dubreuil-Tranchant,1 Romain Guyot,1 Amira Guellim,1 Caroline Duret,1, 2 1 1, 3 ´ 1 1 1 Marion de la Mare, Norosoa Razafinarivo, Valerie Poncet, Serge Hamon, Perla Hamon, and Alexandre de Kochko1 1 IRD, UMR DIADE, Centre IRD de Montpellier, BP 64501, 34394 Montpellier Cedex 5, France 2 UMR ECOFOG INRA, BP 709, 97387 Kourou Cedex, Guyane Franc¸aise, France 3 FOFIFA, BP 1444, Ambatobe, Antananarivo 101, Madagascar Correspondence should be addressed to Alexandre de Kochko, dekochko@ird.fr Received 1 March 2011; Accepted 16 July 2011 Academic Editor: A. Rus Hoelzel Copyright © 2011 Christine Dubreuil-Tranchant et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Miniature Inverted-repeat Transposable Elements (MITEs) are small nonautonomous class-II transposable elements distributed throughout eukaryotic genomes. We identified a novel family of MITEs (named Alex ) in the Coff ea canephora genome often associated with expressed sequences. The Alex-1 element is inserted in an intron of a gene at the CcEIN4 locus. Its mobility was demonstrated by sequencing the insertion site in C. canephora accessions and Coff ea species. Analysis of the