不同膳食脂肪酸构成对大鼠肝脏hmg-coar-第三军医大学学报.docVIP

不同脂肪酸构成对大鼠肝脏HMG-CoAR、SREBP-1cHMG-CoAR、SREBP-1cmRNA表达的影响。方法 雌性SD大鼠随机分成6组喂养6种不同膳食脂肪酸组成的饲料，即饱和脂肪酸组(SFA)、单不饱和脂肪酸组(MUFA)、n-6多不饱和脂肪酸组(n-6 PUFA)、n-3n-3 PUFA)、1:1 n-6／n-318w。并在实验8w和18w提取大鼠肝脏组织，利用 RT- PCR技术检测大鼠肝脏组织中HMG-CoAR、SREBP-1c mRNAHMG-CoAR、SREBP-1cSFA、MUFAHMG-CoAR途径来调控机体的脂质代谢，它们对SREBP-1c的表达无明显影响。其中，与对照组相比较，SFA能非常显著地升高HMG-CoAR mRNA的表达(P＜0.01)，而MUFA能显著降低 HMG-CoAR的基因表达(P＜0.05)。PUFA能够显著降低HMG-CoAR及SREBP-1c的表达。 关键词：n-6PUFA；n-3PUFA脂质代谢；HMG-CoAR；SREBP-1c 中图法分类号： 文章标识码： Study on the effect of different fatty acid compositions on gene’s mRNA HMG-CoAR SREBP-1c of rat liver Shi jiao-jiao, MI Man-tian, WEI Na， WANG Bin (Department of Nutrition and Food Hygiene The Third Military Medical University,Chongqing key laboratory of Nutrition and Food Satety, Chongqing 400038, China) [Abstract] Objective: To study the effect of different dietary fatty acid compositions on gene’s mRNA HMG-CoAR SREBP-1c of rat liver. Method: Female SD rats were fed diets containing 15％fat(wt／wt)in the experimental period．he rats were randomly assigned into 6 groups fed 6 different diets respectively, containing following fatty acids: saturated fatty acid(SFA), monounsaturated fatty acid(MUFA) , n-6 polyunsaturated fatty acid(PUFA) , n-3 PUFA, 1:1 n-6／n-3, and control the groups for 18w．Use the RT-PCR method examined to test each ’ rat liver organization of 8 weeks and 18 weeks lipid metabolism of the key gene’s mRNA such as: HMG-CoAR, SREBP-1c. Results: Detection, the SFA, MUFA mainly is the lipid metabolism that passes HMG-CoAR path to adjust to control the body, their expression to SREBP-1c has no obvious influence. Among them, compared with control control, SFA can show the ground expression (P0.01) that goes up the HMG-CoARmRNA very much, but the MUFA can show the gene expression that the lowers HMG-CoAR(P0.05). For the PUFA, they regulate the effect of the blood fat more overall, can show to lower the lipoids synthesize of key enzymeHMG-CoAR and the expression of the SREBP-1