表皮干细胞标记物在仿刺参正常和再生体壁中的表达研究-农学学报.pdfVIP

农学学报 2012,2(04):50-55 Journal of Agriculture 表皮干细胞标记物在仿刺参正常 和再生体壁中的表达研究 李 霞，王微微，李 强，秦艳杰 （大连海洋大学/辽宁省海洋生物资源与生境恢复重点实验室，辽宁大连116023） 摘 要：为探究仿刺参表皮再生的机制和再生修复过程中的表皮细胞来源，采用免疫荧光方法，观察β1 整合素，α6 整合素，角蛋白19 (ck19) 3 种表皮干细胞标记物在仿刺参正常及创伤后再生不同时间段的 体壁中的表达情况。结果表明，ck19在对照组和试验组仿刺参体壁中均呈阳性，并且随着再生的进行， 阳性细胞数量和分布区域均有变化。创伤第1 天阳性细胞向创伤面上缘迁移，细胞数量同对照组；第3 天阳性细胞数量显著下降；第5 天阳性细胞数量最多；第7 天阳性细胞数量有所下降；11 天、13 天阳性细 胞的数量较第7 天稍有增加；第19 天阳性细胞数量同对照组，但排列很分散。β1整合素和α6 整合素在 正常及创伤后再生的体壁中均未见阳性信号出现。结合石蜡切片观察结果进行比较分析认为被ck19 标记的细胞是参与仿刺参体壁表皮再生的干细胞和短暂扩增细胞，仿刺参表皮再生的机制属于新建再 生。 关键词：仿刺参；体壁；间接免疫荧光；β1整合素；α6 整合素；角蛋白19(ck19) 中图分类号：Q291 文献标志码：A 论文编号：2011-0869 Studies on the Expression of Identification Makers of Epidermal Stem Cell in the Natural and Regenerate Body Wall of Apostichopus j aponicus Li Xia, Wang Weiwei, Li Qiang, Qin Yanjie (Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, Liaoning, China) Abstract: In order to study the mechanism of epidermis regeneration in A. j aponicus, and the origination of epidermal cells.The expressions of identification markers of stem cells, the β1 integin, α6 integin and keratin 19(ck19), were studied in the natural and regenerate body wall of sea cucumber through Immunofluorescence method. Results showed that ck19 expression was positive both in the control group and the experimental group. At 1 day of wounding, the cells which expressed ck 19 moved towards the edges of the wound surface, and the number of these kinds of cells were similar to those in the control group. At 3 days of wounding, the