革兰阳性球菌1914株耐药性监测.PDFVIP

中国杭感染化厅杂志2004年2月28日第4卷第1期ChinIInfectChen,other,Feb.2004,Vol.4,No.1 ·论 著 · 革兰阳性球菌 1914株耐药性监测 朱德妹 汪 复 吴 提 胡付品 张婴元 仁摘要] 目的:了解上海地区临床分离革兰阳性球菌对常用抗菌药物的敏感性。方法:Kirby-Bauer法检测2002年3-5月上 海10所医院1914株临床分离革兰阳性球菌对20种抗菌药物的耐药性，并采用微量稀释法、琼脂平板稀释法和E试验对部 分菌株进行万古霉素和替考拉宁的最低抑菌浓度(M工C)测定。结果:替考拉宁和万古霉素抗菌活性最强，只有2株细菌(1株 溶血葡萄球菌和飞株华纳葡萄球菌)对替考拉宁中介 E〔试验)。无替考拉宁和万古霉素耐药株(稀释法) 结论;替考拉宁和 万古霉素对革兰阳性球菌的抗菌活性最强，两者的抗菌活性相似。临床微生物实验室在遇到K-B法结果为替考拉宁和万古 霉素中介或耐药时，必须采用稀释法或E试验以确证其是否耐药 [关键词〕革兰阳性球菌;替考拉宁;万古霉素 中图分类号:R378.1 文献标识码:A 文寒编号:1009-7708(2004)01-0001-05 SurveillanceofbacterialresistanceinGr ositivecoed ZHUDemei， WANGFu, WUShi,HUFupin, ZHANGYingyuan. (InstituteofAntibiotics, HuashanHospital,FudanUniversity,Shanghai200040,China) [Abstract]Objective;ToinvestigatethesusceptibilityofclinicalisolatesofGram-positivecocciinShanghaihospitals.Meth- ads:Bacterialsusceptibilitytestingwascarriedouton1914strainsofGram-positivecocciisolatedfromtenhospitalsinShang- hatfromMarchtoMay2002usingKirby-Baueymethods.20differenttypesofantibioticdisksincludingteicoplaninandvanco mycinwereused.MICaofvancomycinandteicoplaninweredeterminedonsomeofthestrainsbymicrodilution,agardilution andEtestmethods.Results;BothteicoplaninandvancomycinwerethemostactiveagentsagainstGram-positivecocci,onlyone strainofStaphylococcushaemolyticusandS.tuamerirespectivelywerefoundtobeintermediatesusceptibletoteicoplaninbyE test,nostrainsresistanttoteicoplaninandvancomycinwerefoundbydilutionmethods.Conclusions;Bothteicoplaninandvan- comycinarethemostactiveagentsagainstGram-positivecacti.Strainsnonsusceptibletoteicoplaninorvancomycindetermined byKirby-BauermethodshouldbeconfirmedbyEtestordilutionmethods. 仁Keywords口Gram-positivecocci,Teicoplanin;Vancomycin 替考拉宁(teicoplanin)是一种糖肤类抗生素， 10所医院的临床分离株，其中1所医院为2002