复合菌剂对有机磷农药毒害小麦的缓解作用.docVIP

复合菌剂对有机磷农药毒害小麦的缓解作用 摘要：采用水培法研究不同浓度的（106cfu/mL、107cfu/mL、108cfu/mL）复合菌剂及其施用方法与有机磷农药的复合作用对小麦毒性的影响。结果表明：106～108 cfu/mL浓度的复合菌剂对有机磷农药的毒害均有缓解作用，小麦的根长、株高、茎粗、干重、湿重、叶绿素含量、POD、SOD和CAT的活性均有不同程度的提高，而MDA含量下降。但不同浓度的复合菌剂作用效果不同，107 cfu/mL的复合菌剂可显著缓解有机磷农药对小麦株高的抑制作用；108 cfu/mL的复合菌剂对有机磷农药毒害小麦的根长、茎粗及抗氧化酶活性的缓解作用最显著。同一浓度的复合细菌的不同施用方法对有机磷农药毒性的缓解作用存在差异。6795 关键词：复合菌剂；有机磷农药；叶绿素含量；抗氧化酶活性；MDA含量 Relieving to Organophosphorus Pesticide Injury of Wheat by Complex Bacterium Abstract: The combined effects of organophosphorus pesticide, the different concentrations ( 106cfu/mL,107cfu/mL,108cfu/mL) and different application methods of complex bacterium on wheat were studied, respectively. The results showed that the different concentrations of complex bacterium can alleviate the injury of organophosphorus pesticide on wheat to various degree. Complex bacterium can increase remarkably the length of root, plant height, stem diameter, dry weight, wet weight, content of chlorophyll and the activities of POD, SOD and CAT. The content of MDA was significantly decreased. The complex bacterium can obviously relieve organophosphorus pesticide restraint on the height of wheat at the concentration of 107cfu/mL. The complex bacterium can increase significantly the length of root,stem diameter, content of chlorophyll and the activities of antioxidant enzymes at the concentration of 108cfu/mL. The relieving effects of complex bacterium are different among two application methods of complex bacterium. Key words: Complex bacterium; Organophosphorus pesticide; Chlorophyll content; Activities of antioxidant enzymes; MDA content 光合细菌是一种以光为能源，CO2和有机物为碳源，以有机物、氢气或硫化物为供氢体的原核生物的总称。光合细菌制品作为一种现代生物技术产品已被广泛应用于菌肥、饲料添加剂，都取得了较好的抗病、防病效果[4]。杨绍斌等[5]报道了复合菌剂可提高蔬菜绿色食品的产量。有关复合菌剂对过量有机磷农药毒害作物是否有缓解作用，以及复合菌剂的施用方法对缓解作用影响的研究，尚未见报道。本文以小麦为材料，研究不同浓度的复合菌剂和有机磷农药复合处理对小麦的生长指标和生理生化指标的影响，并对复合菌剂施用方式进行研究，以探求复合菌剂对作物抗有机磷农药毒害的作用机理，为农业生产中合理使用菌肥、缓解过量有机磷农药对作物产生的不良影响，提供方法和理论依据。 1 材料与方法 1.1 试验材料 供试小麦，由周口师范学院生命科学系提供。复合菌剂来源于微生物实验室，经活化、培养、稀释成浓度分别为106cfu/mL、1