怀牛膝茎叶多糖研究及生物活性研究.docVIP

怀牛膝茎叶多糖研究及生物活性研究 摘要：本实验通过微波辅助提取和热水浸提两种方法，提取怀牛膝茎叶多糖，并测定样品提取液对农杆菌、大肠杆菌的抑菌作用，同时研究其抗氧化活性。结果表明，微波辅助提取和热水浸提法对怀牛膝茎叶多糖提取率分别为119.59 mg/g、193.79 mg/g。热水浸提液对农杆菌及大肠杆菌抑菌效果较好，抑菌圈直径均为7.13 mm。样品提取液有明显的抗氧化活性，其中热水浸提液对超氧阴离子自由基的清除率为77.38％，对羟自由基的清除率为48.24％。 关键词：怀牛膝；多糖；生物活性；抗氧化；抑菌 Study on the Polysaccharide Extraction from Stems and Leaves of Achyranthes Bidentata and Its Bioactivity Abstract: In this experiment, the polysaccharide was extracted by microwave- assisted and hot-water extraction from Stems and Leaves of Achyranthes bidentata. The antibacterial effects on bacterials such as Escherichia coli and