关于四氯化碳肝损伤的组织病理学诊断方法的探讨 - 中国实验动物学报.doc

对胃粘膜损伤有辅助保护功能的评价试验中的病理学方法的探讨与建议 金毅，甘玉玺2，刘远平1，陈润桦1，谢天柱1，黄泽愉1 （1. 深圳市药品检验所深圳药品质量标准研究重点实验室，广东 深圳 518057；2. 中兴通讯股份有限公司，广东 深圳 518055） [摘要] 目的 建立科学的大体病理学检查和组织病理学诊断的评分原则、更合理的评分标准以及合理的统计学方法，为保健食品对胃粘膜损伤有辅助保护作用提供更为科学、可行的评价方法。方法 通过对大鼠胃黏膜急性损伤酒精模型的全面分析，针对现行方法中评价方法的缺点，根据病变特点及病理学检查的原则和半定量分析方法，以及相应的统计学方法等信息化处理，进行方法学探讨。结果 大体病理学检查按照病变所占面积以及在整个胃粘膜所占的比例；组织病理学诊断根据病变累及粘膜层的深度作为判定粘膜受损严重程度的主要依据，其他各种病变作为参考因素。分别分为未见异常（0分）、轻度病变（1分）、中度病变（2分）和重度病变（3分）。统计学方法采用Ridit统计学方法分析病理学半定量的结果。结论 从大体病理学、组织病理学的评价方法以及数据处理方法和结果判定等方面，提出了对胃粘膜损伤有辅助保护功能的动物实验相对更为科学、可行的评价方法的建议。 [关键词] 病理学；评价方法；急性胃黏膜损伤酒精模型；信息化 Pathologic Study and Suggestion on Evaluation Methods of Auxiliary Protective Function on Gastric Mucosa Injury JIN Yi1, GAN Yu-xi2, LIU Yuan-ping1, CHEN Run-hua1, XIE Tian-zhu1, HUANG Ze-yu1 (1. Shenzhen Key Laboratory of Drug Quality Standard Research, Shenzhen Institute for Drug Control, Guangdong Shenzhen 518057, China; 2. ZTE Corporation, Guangdong Shenzhen 518057, China) [Abstract] Objective To establish a scientific and practical principle, grading standard and reasonable statistical method for evaluating the protective effect of health food to gastric mucosal injury, based on general pathology and histopathological diagnosis. Methods A methodological study was conducted on rat model of acute gastric mucosa injury induced by alcoholic through comprehensive analysis and comparing shortcomings of the current standard evaluation method, and methods of semi-quantitative analysis and corresponding information statistic processing were based on characteristics of the lesion and principles of pathology. Results Gross pathological evaluation of gastric mucosa lesion was based on the area occupied and proportion in the whole gastric mucosa. Histopathological diagnosis was based on the mucous layer depth of lesion as main determination point and other lesions as reference factors. Grades of lesion were divided into no abnormality (0), mild lesions (1 point), moderate lesions (2 points), and severe lesions (3 points).