益母草抗感染镇痛作用实验探究.docVIP

益母草抗感染镇痛作用实验探究[摘要] 目的:观察益母草的抗感染镇痛作用。方法:采用热板法和二甲苯耳廓肿胀法观察益母草对小鼠镇痛和抗感染作用的效果。结果:益母草给药组小鼠耳廓肿胀与模型组比较,差异无统计学意义(P0.05);益母草给药组小鼠疼痛反应潜伏期有所延长,但差异无统计学意义(P0.05)。结论:益母草无明显抗感染作用,有一定的镇痛作用。 [关键词] 益母草;镇痛作用;抗感染作用 [中图分类号] R285.5[文献标识码]A [文章编号]1673-7210(2010)03(a)-027-02 Experimental study on the anti-inflammatory and analgesic effects of Herba Leonuri ZHANG Yuewen1, SUN Shuguang2, GAO Chunlian2, LIU Ruili2, MA Ruijuan2, CHEN Yuanpeng2, WEI Rongrui2 (1.Zhejiang Chinese Medical University, Hangzhou 310053, China; 2.He’nan College of Traditional Chinese Medicine, Zhengzhou 450008, China) [Abstract] Objective: To study the anti-inflammatory and analgesic effects of Herba Leonufi. Methods: The analgesic effect and the anti-inflammatory effect of Herba Leonufi were observed with hotplate and ear swelling induced by xylen method. Results: There was no significant difference in ear swelling induced by xylen between the group with Herba Leonuri and control group (P0.05). Group with Herba Leonuri prolonged pain latency in mice, but there was no significant difference compared with the control group (P0.05). Conclusion: Herba Leonuri has no significant anti-inflammatory effect, but it has analgesic effect in some tendency. [Key words] Herba Leonuri; Analgesic effect; Anti-inflammatory effect 益母草(Herba Leonuri)为唇形科植物,味苦、辛,性微寒,归肝、心包经,全草入药,具有活血祛瘀、调经及利水消肿之功效,为妇科经产要药,主要具有兴奋子宫[1]、改善血流动力学[2]、保护缺血心肌[3]、美容[4]等药理作用。本实验主要观察益母草对小鼠抗感染及镇痛方面的作用。 1 材料与方法 1.1 试药 益母草,市售;二甲苯,天津市化学试剂三厂生产(批号:070528);电子天平,FA(N)/JA(N),上海民桥精密仪器有限公司生产。 1.2 动物 昆明种小鼠60只,体重18～20 g,由河南中医学院机能实验室提供。 1.3 方法 1.3.1 镇痛将小鼠随机分成四组:空白对照组,益母草低剂量组,益母草中剂量组,益母草高剂量组,每组12只,给药组分别给予低剂量(相当于原生药2.1 g/kg)、中剂量(4.2 g/kg)、高剂量(6.3 g/kg)益母草,每天给药1次,连续给药4 d。于末次给药后30 min热板法测定小鼠疼痛反应潜伏期(即观察小鼠首次舔后足时间),观察益母草对小鼠镇痛作用的影响。 1.3.2 抗感染将小鼠随机分成五组:空白对照组,模型组(二甲苯),益母草低剂量组,益母草中剂量组,益母草高剂量组,每组12只,给药剂量同“1.3.1”项下方法,每天给药1次,连续给药4 d,末次给药后60 min将二甲苯均匀涂在小鼠右耳的前后两面,每只0.02 ml,致感染后4 h将小鼠处死,剪下两耳,并将两耳重叠用打孔器(9 mm)打孔,再用分析天平称取耳片的重量,计算各组小鼠的耳片肿胀程度。耳片肿胀程度=致感染侧耳