绿茶提取物表没食子儿茶素没食子酸酯对酸蚀症牙本质胶原降解作用初步探究.docVIP

绿茶提取物表没食子儿茶素没食子酸酯对酸蚀症牙本质胶原降解作用初步探究[摘要] 目的 观察绿茶提取物表没食子儿茶素没食子酸酯（EGCG）对酸蚀症暴露的牙本质胶原降解作用的影响。方法 选取酸蚀症志愿者64名，随机分成两组。制作个别托盘，托盘内注入羧甲基纤维素钠（CMC）凝胶（对照组）或添加了EGCG的CMC凝胶（试验组）。要求志愿者于夜间睡时佩戴，清晨取下。试验前1 d，以及试验后第1、2、3、4 周收集培养液，用放射免疫试剂盒法测量Ⅰ型胶原羧基端肽（ICTP）的质量浓度。结果 凝胶种类和使用时间对 ICTP的质量浓度均有影响；EGCG能快速、有效地降低ICTP的表达水平，与对照组相比，差异有统计学意义（P0.05）。结论 EGCG能有效地减少脱矿牙本质胶原的降解，可以提高牙本质抗酸蚀的能力。 [关键词] 表没食子儿茶素没食子酸酯； 牙本质； 胶原 [中图分类号] R 781.2 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.05.023 Pilot study of the effect of green tea extractive epigallocatechin-3-gallate on degradation of collagen in dental erosion Chen Huangqin, Huang Bin. （Dept. of Stomatology, Hubei University of Science and Technology, Xianning 437100, China） [Abstract] Objective To observe the effect of green tea extractive epigallocatechin-3-gallate（EGCG） on degra- dation of collagen in dental erosion. Methods Sixty-four volunteers with dental erosion were randomly divided into two groups. The custom fitted trays were made from the heat curable braces and were injected with carboxymethyl cellulose sodium（CMC） hydrogel in one group（control group） or CMC hydrogel with EGCG added in another（experi-mental group）. These trays were used during sleep and removed from oral cavity in the next morning. The content of carboxy-terminal telopeptide of type Ⅰ collagen（ICTP） were determined by radioimmunoassay kit at 1 d before the experiment, and 1, 2, 3, 4 weeks after the experiment. Results Both the types and time showed significant effects on ICTP. The incubation in EGCG resulted in relatively rapid and significant（P0.05） decrease in the expression level of ICTP compared with the control group. Conclusion EGCG can inhibit collagen degradation and improve the anti-erosive effect of dentine. [Key words] epigallocatechin-3-gallate； dentine； collagen 牙齿酸蚀症是指在没有细菌参与的情况下，由于酸的化学侵蚀或螯合作用，牙体表面硬组织局部性、病理性、慢性进行性丧失的一种疾病[1]。釉质酸 蚀表现为一种向心性的矿物质溶解过程，直至暴露釉质下方的牙本质。脱矿牙本质中，有机质的存在能阻挡离子的扩散，减少酸蚀底物的丧失[2]，并能保 护剩余的脱矿牙本质抵挡刷牙等机械性磨损[3]。基质 金属蛋白酶（matrix metallopr