转OsCYP2基因水稻芽期和幼苗前期抗盐碱性鉴定.docVIP

转OsCYP2基因水稻芽期和幼苗前期抗盐碱性鉴定摘要：以0.15%浓度Na2CO3模拟盐碱胁迫，对转OsCYP2基因水稻吉农大30T3代种子进行芽期与幼苗前期抗盐碱性鉴定研究，结果表明，转基因材料根长与苗高比对照吉农大30低；无论是转基因水稻还是非转基因水稻，其幼苗前期对于盐碱胁迫的危害比芽期更为敏感；在0.15% Na2CO3胁迫下转基因材料与对照材料各项性状测量指标与芽期综合相对碱害率间没有显著差异，但是转基因吉农大30幼苗前期综合相对碱害率与对照相比发生了显著降低，说明转基因材料在0.15%Na2CO3胁迫下其幼苗受到的伤害少于对照材料，由此推断转基因材料幼苗前期耐盐碱能力高于对照材料。 关键词：OsCYP2基因；抗盐碱性鉴定；发芽期；幼苗前期 中图分类号：S511 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2012.05.003 Saline—Alkaline Tolerance Test of OsCYP2 Transgenic Rice in Gremination Period and Early Seedling Stage YAN Hai—sheng，MA Jing—yong （Jilin Agricultural University ，Changchun，Jilin 130118，China） Abstract： The saline—alkaline tolerance of OsCYP2 transgenic rice by using Na2CO3 at 0.15% concentration as saline—alkaline stress in the germination period and early seedling stage was studied ， experiments showed that the length of root and height of seedling of transgenic rice was lower compared with the control Jinongda 30 ； both transgenic rice and ordinary rice is sensitive to the saline—alkaline stress in their early seedling stage than the germination period ； there was no significance different appears of the character metrics and total value of relative alkaline damage rate of rice germinative period between transgenic rice and ordinary rice ， but the total value of relative alkaline damage rate of rice early seedling stage of transgenic Jinongda 30 was significantly reduced compared with control ， so that the saline—alkaline tolerance of trancegenic rice in the early seedling stage was stronger than that of control. Key words： OsCYP2 gene ； saline—alkaline tolerance test ； germination ； early seedling stage 盐碱胁迫极大地影响了盐碱地区作物生长，造成作物减产，盐碱土的相关性质以及改造盐碱土，培育适宜盐碱地区生长的作物品种一直是科技工作者研究的重点之一。水稻耐盐育种工作中耐盐碱筛选的方法与标准对于筛选结果至关重要，目前筛选鉴定方法主要有实验室鉴定、温室鉴定、人工田间和自然田间鉴定等方法，通过发芽指标、形态伤害评价、生长量比较以及田间农艺性状比较等方法对植物耐盐性进行评价，相比于田间种植的方法，在实验室内通过发芽试验进行筛选更容易控制试验条件，具有操作性较强、周期短、效率高的优点，适宜于初期进行耐盐种质的筛选，可以快速缩小所需鉴定材料的范围；对于筛选的标准，一般采用发芽势与发芽率的差别进行鉴定，同时对于耐盐性鉴定所使用的胁迫浓度，有研究认为0.15%～0.2%浓度Na2CO3胁迫适宜于进行芽期与幼苗前期的抗盐性筛选。 通过分