ataxin-2-like is a regulator of stress granules and processing bodiesataxin-2-like监管机构的压力颗粒和处理尸体.pdfVIP

Ataxin-2-Like Is a Regulator of Stress Granules and Processing Bodies 1,4 ¨ 2,5 1 1¤ 2,5 3 Christian Kaehler , Jorg Isensee , Ute Nonhoff , Markus Terrey , Tim Hucho , Hans Lehrach , Sylvia Krobitsch1* 1 Otto Warburg Laboratory, Max Planck Institute for Molecular Genetics, Berlin, Germany, 2 Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Berlin, Germany, 3 Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany, 4 Free University Berlin, Department of ¨ ¨ ¨ ¨ ¨ Biology, Chemistry and Pharmacy, Berlin, Germany, 5 Universitatsklinik Koln, Klinik fur Anasthesiologie und Operative Intensivmedizin, Experimentelle Anasthesiologie und Schmerzforschung, Cologne, Germany Abstract Paralogs for several proteins implicated in neurodegenerative disorders have been identified and explored to further facilitate the identification of molecular mechanisms contributing to disease pathogenesis. For the disease-causing protein in spinocerebellar ataxia type 2, ataxin-2, a paralog of unknown function, termed ataxin-2-like, has been described. We discovered that ataxin-2-like associates with known interaction partners of ataxin-2, the RNA helicase DDX6 and the poly(A)- binding protein, and with ataxin-2 itself. Furthermore, we found that ataxin-2-like is a component of stress granules. Interestingly, sole ataxin-2-like overexpression led to the induction of stress granules, while a reduction of stress granules was detected in case of a low ataxin-2-like level. Fi