basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility斑马鱼basonuclin-2要求成人色素模式发展和女性生育能力.pdfVIP

Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility 1.¤ 1. 1. 2 Michael R. Lang , Larissa B. Patterson , Tiffany N. Gordon , Stephen L. Johnson , David M. Parichy1* 1 Department of Biology, University of Washington, Seattle, Washington, United States of America, 2 Department of Genetics, Washington University School of Medicine, St. Louis, Missouri, United States of America Abstract Relatively little is known about the generation of adult form. One complex adult trait that is particularly amenable to genetic and experimental analysis is the zebrafish pigment pattern, which undergoes extensive remodeling during post-embryonic development to form adult stripes. These stripes result from the arrangement of three classes of neural crest-derived pigment cells, or chromatophores: melanophores, xanthophores, and iridophores. Here, we analyze the zebrafish bonaparte mutant, which has a normal early pigment pattern but exhibits a severe disruption to the adult stripe pattern. We show that the bonaparte mutant phenotype arises from mutations in basonuclin-2 (bnc2), encoding a highly conserved, nuclear- localized zinc finger protein of unknown function. We show that bnc2 acts non-autonomously to the melanophore lineage and is expressed by hypodermal cells adjacent to chromatophores during adult pigment pattern formation. In bonaparte (bnc2) mutants, all three types of chromatophores differentiate but then are lost by extrusion through the skin. We further show that while bnc2 promotes the development of two genetically distinct populations of melanophores in the body stripes, chromatophores of the fins and scales remain unaffected in bon