co-inoculation with rhizobia and amf inhibited soybean red crown rot from field study to plant defense-related gene expression analysisco-inoculation根瘤菌和amf抑制大豆红冠腐病从野外研究植物与国防有关的基因表达分析.pdfVIP

Co-Inoculation with Rhizobia and AMF Inhibited Soybean Red Crown Rot: From Field Study to Plant Defense- Related Gene Expression Analysis 1 1 1 1 1,2 1 3 1 Xiang Gao , Xing Lu , Man Wu , Haiyan Zhang , Ruqian Pan , Jiang Tian , Shuxian Li , Hong Liao * 1 State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Root Biology Center, South China Agricultural University, Guangzhou, China, 2 Laboratory of Bacteria and Fungicides, South China Agricultural University, Guangzhou, China, 3 Crop Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service, Stoneville, Mississippi, United States of America Abstract Background: Soybean red crown rot is a major soil-borne disease all over the world, which severely affects soybean production. Efficient and sustainable methods are strongly desired to control the soil-borne diseases. Principal Findings: We firstly investigated the disease incidence and index of soybean red crown rot under different phosphorus (P) additions in field and found that the natural inoculation of rhizobia and arbuscular mycorrhizal fungi (AMF) could affect soybean red crown rot, particularly without P addition. Further studies in sand culture experiments showed that inoculation with rhizobia or AMF significantly decreased severity and incidence of soybean red crown rot, especially for co- inoculation with rhizobia and AMF at low P. The root colony forming unit (CFU) decreased over 50% when inoculated by rhizobia and/or AMF at low P. However, P addition only enhanced CFU when inoculated with AMF. Furthermore, root exudates of soybean inoculated with rhizobia and/or AMF significant