comparative analysis of zinc finger proteins involved in plant disease resistance比较分析锌指蛋白参与植物的抗病性.pdfVIP

Comparative Analysis of Zinc Finger Proteins Involved in Plant Disease Resistance 1,2 1,2 2 1 Santosh Kumar Gupta , Amit Kumar Rai , Shamsher Singh Kanwar , Tilak R. Sharma * 1 National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India, 2 Department of Biotechnology, Himachal Pradesh University, Summer-Hill, Shimla, India Abstract A meta-analysis was performed to understand the role of zinc finger domains in proteins of resistance (R) genes cloned from different crops. We analyzed protein sequences of seventy R genes of various crops in which twenty six proteins were found to have zinc finger domains along with nucleotide binding sites - leucine rice repeats (NBS-LRR) domains. We identified thirty four zinc finger domains in the R proteins of nine crops and were grouped into 19 types of zinc fingers. The size of individual zinc finger domain within the R genes varied from 11 to 84 amino acids, whereas the size of proteins containing these domains varied from 263 to 1305 amino acids. The biophysical analysis revealed that molecular weight of Pi54 zinc finger was lowest whereas the highest one was found in rice Pib zinc finger named as Transposes Transcription Factor (TTF). The instability (R2 = 0.95) and the aliphatic (R2 = 0.94) indices profile of zinc finger domains follows the polynomial distribution pattern. The pairwise identity analysis showed that the Lin11, Isl-1 Mec-3 (LIM) zinc finger domain of rice blast resistance protein pi21 have 12.3% similarity with the nuclear transcription factor, X-box binding-like 1 (NFX) type zinc finger domain of Pi54 protein. For the first time, we reported that Pi54 (Pi-kh-Tetep), a rice blast resistance (R) pr