circadian clocks are resounding in peripheral tissues生物钟是响亮的外围组织.pdfVIP

Circadian Clocks Are Resounding in Peripheral Tissues 1 2 3 3 1 2,4* Andrey A. Ptitsyn , Sanjin Zvonic , Steven A. Conrad , L. Keith Scott , Randall L. Mynatt , Jeffrey M. Gimble 1 Experimental Obesity Laboratory, Louisiana State University Pennington Biomedical Research Center, Baton Rouge, Louisiana, United States of America, 2 Stem Cell Laboratory, Louisiana State University Pennington Biomedical Research Center, Baton Rouge, Louisiana, United States of America, 3 Departments of Bioinformatics and Computational Biology, Medicine, and Emergency Medicine, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States of America, 4 Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, United States of America Circadian rhythms are prevalent in most organisms. Even the smallest disturbances in the orchestration of circadian gene expression patterns among different tissues can result in functional asynchrony, at the organism level, and may to contribute to a wide range of physiologic disorders. It has been reported that as many as 5%–10% of transcribed genes in peripheral tissues follow a circadian expression pattern. We have conducted a comprehensive study of circadian gene expression on a large dataset representing three different peripheral tissues. The data have been produced in a large-scale microarray experiment covering replicate daily cycles in murine white and brown adipose tissues as well as in liver. We have applied three alternative algorithmic approaches to identify circadian oscillation in time series expression profiles. Analyses of our own data indicate that the expression of at least 7% to 21% of active genes in mouse liver, and in white a