comprehensive analysis reveals dynamic and evolutionary plasticity of rab gtpases and membrane traffic in tetrahymena thermophila综合分析揭示了动态和进化的可塑性rab gtpase和膜的交通四膜虫thermophila.pdfVIP

Comprehensive Analysis Reveals Dynamic and Evolutionary Plasticity of Rab GTPases and Membrane Traffic in Tetrahymena thermophila 1 1 1 2 1 Lydia J. Bright , Nichole Kambesis , Scott Brent Nelson , Byeongmoon Jeong , Aaron P. Turkewitz * 1 Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United States of America, 2 Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Korea Abstract Cellular sophistication is not exclusive to multicellular organisms, and unicellular eukaryotes can resemble differentiated animal cells in their complex network of membrane-bound structures. These comparisons can be illuminated by genome- wide surveys of key gene families. We report a systematic analysis of Rabs in a complex unicellular Ciliate, including gene prediction and phylogenetic clustering, expression profiling based on public data, and Green Fluorescent Protein (GFP) tagging. Rabs are monomeric GTPases that regulate membrane traffic. Because Rabs act as compartment-specific determinants, the number of Rabs in an organism reflects intracellular complexity. The Tetrahymena Rab family is similar in size to that in humans and includes both expansions in conserved Rab clades as well as many divergent Rabs. Importantly, more than 90% of Rabs are expressed concurrently in growing cells, while only a small subset appears specialized for other conditions. By localizing most Rabs in living cells, we could assign the majority to specific compartments. These results validated most phylogenetic assignments, but also indicated that some sequence-conserved Rabs were co-opted for novel functions. Our survey uncov