cord blood cd4+ t cells respond to self heat shock protein 60 (hsp60)脐带血cd4 + t细胞应对自我热休克蛋白60(hsp60).pdfVIP

Cord Blood CD4+ T Cells Respond to Self Heat Shock Protein 60 (HSP60) 1 1 1 1 1 Joost A. Aalberse *, Berber Kapitein , Sytze de Roock , Mark R. Klein , Wilco de Jager , Ruurd van der 2 3 1 1 Zee , Maarten O. Hoekstra , Femke van Wijk , Berent J. Prakken 1 Department of Pediatric Immunology, Wilhelmina Children’s Hospital, Utrecht, The Netherlands, 2 Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands, 3 Department of Pediatrics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands Abstract Background: To prevent harmful autoimmunity most immune responses to self proteins are controlled by central and peripheral tolerance. T cells specific for a limited set of self-proteins such as human heat shock protein 60 (HSP60) may contribute to peripheral tolerance. It is not known whether HSP60-specific T cells are present at birth and thus may play a role in neonatal tolerance. We studied whether self-HSP60 reactive T cells are present in cord blood, and if so, what phenotype these cells have. Methodology/Principal Findings: Cord blood mononuclear cells (CBMC) of healthy, full term neonates (n = 21), were cultured with HSP60 and Tetanus Toxoid (TT) to study antigen specific proliferation, cytokine secretion and up-regulation of surface markers. The functional capacity of HSP60-induced T cells was determined with in vitro suppression assays. Stimulation of CBMC with HSP60 led to CD4+ T cell proliferation and the production of various cytokines, most notably IL-10, Interf