corazonin neurons function in sexually dimorphic circuitry that shape behavioral responses to stress in drosophilacorazonin神经元函数性态的电路,形状在果蝇行为反应压力.pdfVIP

Corazonin Neurons Function in Sexually Dimorphic Circuitry That Shape Behavioral Responses to Stress in Drosophila 1 1 1¤ 2 1 Yan Zhao , Colin A. Bretz , Shane A. Hawksworth , Jay Hirsh , Erik C. Johnson * 1 Department of Biology, Wake Forest University, Winston-Salem, North Carolina, United States of America, 2 Department of Biology, University of Virginia, Charlottesville, Virginia, United States of America Abstract All organisms are confronted with dynamic environmental changes that challenge homeostasis, which is the operational definition of stress. Stress produces adaptive behavioral and physiological responses, which, in the Metazoa, are mediated through the actions of various hormones. Based on its associated phenotypes and its expression profiles, a candidate stress hormone in Drosophila is the corazonin neuropeptide. We evaluated the potential roles of corazonin in mediating stress- related changes in target behaviors and physiologies through genetic alteration of corazonin neuronal excitability. Ablation of corazonin neurons confers resistance to metabolic, osmotic, and oxidative stress, as measured by survival. Silencing and activation of corazonin neurons lead to differential lifespan under stress, and these effects showed a strong dependence on sex. Additionally, altered corazonin neuron physiology leads to fundamental differences in locomotor activity, and these effects were also sex-dependent. The dynamics of altered locomotor behavior accompanying stress was likewise altered in flies with altered corazonin neuronal function. We report that corazonin transcript expression is altered under starvation and osmotic stress, and that triglyceride and dopamine levels are equally impacted in corazonin neuronal alterations and thes