deregulation of sucrose-controlled translation of a bzip-type transcription factor results in sucrose accumulation in leaves放松管制的sucrose-controlled翻译bzip-type转录因子结果在叶的蔗糖积累.pdfVIP

Deregulation of Sucrose-Controlled Translation of a bZIP-Type Transcription Factor Results in Sucrose Accumulation in Leaves 1. 1,2. 1¤ 3 1 Sunil Kumar Thalor , Thomas Berberich , Sung Shin Lee , Seung Hwan Yang , XuJun Zhu , Ryozo 4 1 1 Imai , Yoshihiro Takahashi , Tomonobu Kusano * 1 Graduate School of Life Sciences, Tohoku University, Aoba, Sendai, Miyagi, Japan, 2 Biodiversity and Climate Research Center (BiK-F), BioCampus-Westend, Frankfurt, Germany, 3 Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University Science Campus, Namdong, Cheoin-Gu, Yongin, Gyeonggi, Korea, 4 Crop Cold Research Team, National Agricultural Research Center for Hokkaido Region, Toyohira-ku, Sapporo, Japan Abstract Sucrose is known to repress the translation of Arabidopsis thaliana AtbZIP11 transcript which encodes a protein belonging to the group of S (S - stands for small) basic region-leucine zipper (bZIP)-type transcription factor. This repression is called sucrose-induced repression of translation (SIRT). It is mediated through the sucrose-controlled upstream open reading frame (SC-uORF) found in the AtbZIP11 transcript. The SIRT is reported for 4 other genes belonging to the group of S bZIP in Arabidopsis. Tobacco tbz17 is phylogenetically closely related to AtbZIP11 and carries a putative SC-uORF in its 59-leader region. Here we demonstrate that tbz17 exhibits SIRT mediated by its SC-uORF in a manner similar to genes belonging to the S bZIP group of the Arabidopsis genus. Furthermore, constitutive transgenic expression of tbz17 lacking its 59-leader region containing the SC-uORF leads to production o