curcumin-loaded apotransferrin nanoparticles provide efficient cellular uptake and effectively inhibit hiv-1 replication in vitrocurcumin-loaded apotransferrin纳米粒子提供有效的体外细胞摄取和有效地抑制hiv - 1复制.pdfVIP

Curcumin-Loaded Apotransferrin Nanoparticles Provide Efficient Cellular Uptake and Effectively Inhibit HIV-1 Replication In Vitro 1 1 2,3 4 1,2,3 Upendhar Gandapu , R. K. Chaitanya , Golla Kishore , Raju C. Reddy , Anand K. Kondapi * 1 Department of Biotechnology, University of Hyderabad, Hyderabad, India, 2 Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India, 3 Centre for Nanotechnology, University of Hyderabad, Hyderabad, India, 4 Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Emory University, Atlanta, Georgia, United States of America Abstract Background: Curcumin (diferuloylmethane) shows significant activity across a wide spectrum of conditions, but its usefulness is rather limited because of its low bioavailability. Use of nanoparticle formulations to enhance curcumin bioavailability is an emerging area of research. Methodology/Principal Findings: In the present study, curcumin-loaded apotransferrin nanoparticles (nano-curcumin) prepared by sol-oil chemistry and were characterized by electron and atomic force microscopy. Confocal studies and fluorimetric analysis revealed that these particles enter T cells through transferrin-mediated endocytosis. Nano-curcumin releases significant quantities of drug gradually over a fairly long period, ,50% of curcumin still remaining at 6 h of time. In contrast, intracellular soluble curcumin (sol-curcumin) reaches a maximum at 2 h followed by its complete elimination by 4 h. While sol-curcumin (GI50 = 15.6 mM) is twice more toxic than nano-curcumin