design of ph sensitive binding proteins from the hyperthermophilic sso7d scaffold设计的ph敏感结合蛋白hyperthermophilic sso7d脚手架.pdfVIP
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design of ph sensitive binding proteins from the hyperthermophilic sso7d scaffold设计的ph敏感结合蛋白hyperthermophilic sso7d脚手架
Design of pH Sensitive Binding Proteins from the
Hyperthermophilic Sso7d Scaffold
Nimish Gera, Andrew B. Hill, Dalon P. White, Ruben G. Carbonell, Balaji M. Rao*
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, United States of America
Abstract
We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin G (hIgG) (hFc) using two
different strategies – histidine scanning and random mutagenesis. We obtained an hFc-binding protein, Sso7d-hFc, through
mutagenesis of the Sso7d pr
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