development and characterization of a new tilling population of common bread wheat (triticum aestivum l.)人口发展和表征的新耕作共同面包的小麦(小麦l .).pdfVIP

Development and Characterization of a New TILLING Population of Common Bread Wheat (Triticum aestivum L.) 1 1 1 2 1 2 Liang Chen , Linzhou Huang , Donghong Min , Andy Phillips , Shiqiang Wang , Pippa J. Madgwick , Martin A. J. Parry2, Yin-Gang Hu1,3* 1 State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest Agricultural and Forestry University, Yangling, Shaanxi, China, 2 Centre for Crop Genetic Improvement, Department of Plant Science, Rothamsted Research, Harpenden, Herts, United Kingdom, 3 Institute of Water Saving Agriculture in Arid Regions of China, Northwest Agricultural and Forestry University, Yangling, Shaanxi, China Abstract Mutagenesis is an important tool in crop improvement. However, the hexaploid genome of wheat (Triticum aestivum L.) presents problems in identifying desirable genetic changes based on phenotypic screening due to gene redundancy. TILLING (Targeting Induced Local Lesions IN Genomes), a powerful reverse genetic strategy that allows the detection of induced point mutations in individuals of the mutagenized populations, can address the major challenge of linking sequence information to the biological function of genes and can also identify novel variation for crop breeding. Wheat is especially well-suited for TILLING due to the high mutation densities tolerated by polyploids. However, only a few wheat TILLING populations are currently available in the world, which is far from satisfying the requirement of researchers and breeders in different growing environments. In addition, current TILLING screening protoco