developmental localization and methylesterification of pectin epitopes during somatic embryogenesis of banana (musa spp. aaa)发展定位和methylesterification体细胞胚胎发生过程中果胶抗原表位的香蕉(穆萨spp。aaa).pdfVIP

Developmental Localization and Methylesterification of Pectin Epitopes during Somatic Embryogenesis of Banana (Musa spp. AAA) 1 1 1 ˇ 2,3 Chunxiang Xu , Lu Zhao , Xiao Pan , Jozef Samaj * ´ 1 College of Horticulture, South China Agricultural University, Guangzhou, Guangdong, China, 2 Department of Cell Biology, Centre of the Region Hana for ´ Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Olomouc, Czech Republic, 3 Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic Abstract Background: The plant cell walls play an important role in somatic embryogenesis and plant development. Pectins are major chemical components of primary cell walls while homogalacturonan (HG) is the most abundant pectin polysaccharide. Developmental regulation of HG methyl-esterification degree is important for cell adhesion, division and expansion, and in general for proper organ and plant development. Methodology/Principal Findings: Developmental localization of pectic homogalacturonan (HG) epitopes and the (1R4)- b- D-galactan epitope of rhamnogalacturonan I (RG-I) and degree of pectin methyl-esterification (DM) were studied during somatic embryogenesis of banana (Musa spp. AAA). Histological analysis documented all major developmental stages including embryogenic cells (ECs),