developmental regulation of hepatitis b virus biosynthesis by hepatocyte nuclear factor 4α乙型肝炎病毒生物合成的发育调控肝细胞的核因子4α.pdfVIP

Developmental Regulation of Hepatitis B Virus Biosynthesis by Hepatocyte Nuclear Factor 4a 1 1 2 1,2 3 Lie Li , Claudia E. Oropeza , Bruno Sainz, Jr. , Susan L. Uprichard , Frank J. Gonzalez , Alan McLachlan1* 1 Department of Microbiology and Immunology College of Medicine, University of Illinois at Chicago, Chicago, Illinois, United States of America, 2 Department of Medicine, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, United States of America, 3 Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America Abstract The host cellular factors that promote persistent viral infections in vivo are, in general, poorly understood. Utilizing the hepatitis B virus (HBV) transgenic mouse model of chronic infection, we demonstrate that the nuclear receptor, hepatocyte nuclear factor 4a (HNF4a, NR2A1), is essential for viral biosynthesis in the liver. The dependency of HBV transcription on HNF4a links viral biosynthesis and persistence to a developmentally regulated transcription factor essential for host viability. Citation: Li L, Oropeza CE, Sainz B Jr., Uprichard SL, Gonzalez FJ, et al. (2009) Developmental Regulation of Hepatitis B Virus Biosynthesis by Hepatocyte Nuclear Factor 4a. PLoS ONE 4(5): e5489. doi:10.1371/journal.pone.0005489 Editor: Donald E. Ganem, University of California San Francisco, United States of America Received March 24, 2009; Accepted April 15, 2009; Published May 8, 2009 Copyright: 2009 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided