dissecting the genetic components of adaptation of escherichia coli to the mouse gut解剖的遗传组成部分改编的小鼠肠道大肠杆菌.pdfVIP

Dissecting the Genetic Components of Adaptation of Escherichia coli to the Mouse Gut 1,2,3,4* 2,5[ 2,5[ ´ 2,3,4,5[ Antoine Giraud , Safia Arous , Marianne De Paepe , Valerie Gaboriau-Routhiau , 2,5 2,3,4,5 1,2 1,2[ 2,5[ Jean-Christophe Bambou , Sabine Rakotobe , Ariel B. Lindner , Franc¸ois Taddei , Nadine Cerf-Bensussan ´ ´ ´ ´ 1 INSERM, U571, Paris, France, 2 Universite Paris Descartes, Faculte de Medecine Rene Descartes, IFR94, Paris, France, 3 INRA UEPSD, Jouy-en-Josas, France, 4 Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden 5 INSERM, U793, Paris, France, While pleiotropic adaptive mutations are thought to be central for evolution, little is known on the downstream molecular effects allowing adaptation to complex ecologically relevant environments. Here we show that Escherichia coli MG1655 adapts rapidly to the intestine of germ-free mice by single point mutations in EnvZ/OmpR two-component signal transduction system, which controls more than 100 genes. The selective advantage conferred by the mutations that modulate EnvZ/OmpR activities was the result of their independent and additive effects on flagellin expression and permeability. These results obtained in vivo thus suggest that global regulators may have evolved to coordinate activities that need to be fine-tuned simultaneously during adaptation to complex environments and that mutations in such regulators permit adjustment of the boundaries of physiological adaptation when switching between two very distinct environments. Citation: Giraud A, Arous