dissecting phaseolus vulgaris innate immune system against colletotrichum lindemuthianum infection解剖菜豆先天免疫系统对炭疽菌lindemuthianum感染.pdfVIP

Dissecting Phaseolus vulgaris Innate Immune System against Colletotrichum lindemuthianum Infection 1. 2. 1 ´ 2 Paula Rodrigues Oblessuc , Aline Borges , Bablu Chowdhury , Danielle Gregorio Gomes Caldas , Siu 2 3 1 Mui Tsai , Luis Eduardo Aranha Camargo , Maeli Melotto * ˜ 1 Department of Biology, University of Texas, Arlington, Texas, United States of America, 2 CENA, Universidade de Sao Paulo, Piracicaba, SP, Brazil, 3 Departamento de ˜ Fitopatologia, ESALQ, Universidade de Sao Paulo, Piracicaba, SP, Brazil Abstract Background: The genus Colletotrichum is one of the most economically important plant pathogens, causing anthracnose on a wide range of crops including common beans (Phaseolus vulgaris L.). Crop yield can be dramatically decreased depending on the plant cultivar used and the environmental conditions. This study aimed to identify potential genetic components of the bean immune system to provide environmentally friendly control measures against this fungus. Methodology and Principal Findings: As the common bean is not amenable to reverse genetics to explore functionality and its genome is not fully curated, we used putative Arabidopsis orthologs of bean expressed sequence tag (EST) to perform bioinformatic analysis and experimental validation of gene expression to identify common bean genes regulated during the incompatible interaction with C. lindemuthianum. Similar to model pathosystems, Gene Ontology (GO) analysis indicated that hormone biosynthe