distribution of high-risk human papillomavirus genotypes among hiv-negative women with and without cervical intraepithelial neoplasia in south africa艾滋病毒阴性的女性的高危人乳头瘤病毒基因型分布在南非没有宫颈上皮内瘤.pdfVIP

Distribution of High-Risk Human Papillomavirus Genotypes among HIV-Negative Women with and without Cervical Intraepithelial Neoplasia in South Africa 1 2 3 4 3 Alicia C. McDonald , Lynette Denny , Chunhui Wang , Wei-Yann Tsai , Thomas C. Wright Jr , Louise Kuhn1,5* 1 Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, United States of America, 2 Department of Obstetrics and Gynaecology, University of Cape Town, Cape Town, South Africa, 3 Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York, United States of America, 4 Department of Biostatistics, Mailman School of Public Health, New York, New York, United States of America, 5 Gertrude H. Sergievsky Center, College of Physicians and Surgeons, Columbia University, New York, New York, United States of America Abstract Objective: Large studies describing the profile of high-risk Human papillomavirus (hrHPV) genotypes among women in sub- Saharan Africa are lacking. Here we describe the prevalence and distribution of hrHPV genotypes among HIV-negative women in South Africa, with and without cervical intraepithelial neoplasia (CIN). Methods: We report data on 8,050 HIV-negative women, aged 17–65 years, recruited into three sequential studies undertaken in Cape Town, South Africa. Women had no history of previous cervical cancer screening. Cervical samples were tested for hrHPV DNA using the Hybrid Capture 2 (HC2) assay and all positive samples were genotyped using a PCR-based assay (Line Blot). Women underwent colposcopy and biopsy/endocervical curettage to determine CIN status.